Rose Bengal Derivatives for Sonodynamic Treatment

摘要

Amphiphilic derivatives of rose bengal (RB) are synthesized to accumulate it into tumor tissues while maintaining its ability to promote acoustic cavitation. Such derivatives are very important for developing 'sonodynamic treatment' which utilizes the synergistic effects of ultrasound and chemicals. Several kinds of derivatives with alkyl groups were synthesized and one of them were found to be amphiphilic (hydrophilic and lipophilic) while others were either hydrophilic or lipophilic. The amphiphilic derivative reduced the cavitation threshold in water as lower as RB do. Tissue distribution of the derivative in mice were investigated and found that the derivative with alkyl group having more than 10 carbon atoms tends to accumulate in tumor tissues. Then we started therapeutic experiments with tumor bearing mice. In Preliminary experiments, a legion was observed by the combination of the RB derivative and ultrasound (1.0+2.0 MHz, 40 W/cm~2 each 3min) while no clear change was observed with ultrasound alone.