FREQUENCY OF VIRULENT FACTORS IN HEMOLYTIC ESCHERICHIA COLJ ISOLATED FROM PIGS BY USING POLYMERASE CHAIN REACTION

摘要

From porcine Escherichia coli isolates, the differentiation of pathogenic strain from non-pathogenic strains should be demonstrated. Initially observation, E. coli which can produced hemolysis zone on blood agar were candidated to be pathogens. The relationship between genotype of isolates and hemolytic characteristics is still enigmatic. In post-weaning diarrhea and edema disease, genotyping by identification of virulent factors for five fimbrial adhesins [KS8ac, ad(F4), K99(F5), 987P(F6), F41, andF18ab,ac (F107) and four enterotoxins (LT, STa, STb, and SLTII) based on molecular approach, mainly the PCR assay, were demonstrated as fast, specific and practical method. In this study, we purposed to determine the frequency of these virulent factors in hemolytic E. coli isolates from clinical cases of pig diagnosed as having enteric infections.