Neurogenesis in zebraflsh embryo cell cultures

摘要

We have carried out experiments to determine the role of FGF in early neurogenesis in zebrafish embryo cell cultures. In the presence of bFGF the level of krox-20 expression was very low while transcripts of another neurogenesis marker, the "engrailed" gene, en-3, were absent. Moreover, we could not detect transcripts of wnt-1 in cells grown in the presence of bFGF and the expression levels of other early neural markers were lower then in the absence of bFGF. In zebrafish it has been shown that wnt-1 is involved in the formation of the midbrain-hindbrain boundary and may influence differentiation of neural crest cells. Our results suggest that the presence of bFGF suppresses the expression of early neural markers in primary cultures of zebrafish.