ENZYME KINETICS MODEL OF THE BACTERIAL LUCIFERASE REACTIONS FOR BIOSENSOR APPLICATIONS

摘要

NPM simulates the NADH→FMNH_2→ light reaction kinetics and it fits with certain experimental data and provides a meaningful calibration curve. The NPM provides a basis for the modelling and simulation of analyte-specific bacterial bioluminescence ―based biosensors. We are continuing to revise and optimise the NPM for different analytical conditions.