APPLICATION OF TWO DIMENSIONAL (2D) NMR SPECTROSCOPY IN THE STRUCTURAL ANALYSIS OF SELECTED POLYSACCHARIDES

摘要

The basic principles of ID and 2D NMR spectroscopy and the most recent application of 2D NMR in the structural analysis and sequencing of some native polysaccharides are reviewed. Native polysaccharides discussed include wheat p-D glucan, a rhamnogalacturonan from yellow mustard gum and a galactomannan from fenugreek. Because of the rapid development in 2D NMR technology, an integrated approach using 2D NMR correlations is becoming a powerful tool for elucidating the complete structure and sequence of some simple native polysaccharides without the use of chemical modification or methylation analysis.