Studies on Breeding of Transketolase-deficient Mutant and Its Characteristics

摘要

Ion beam bioengineering is a cross-disciplinary subject, which was first started in China. After over 10 years of development, it has seen significant achievements in many fields such as mutation breeding, plant gene transfer, life origin and evolution, environmental radiation and human health. Several new fields in theory and application have opened up, offering a new method for genetic engineering and related domains. With its development, it will also play an important role in cell processes and non-equal cell fusion. Taking Bacillus pianilus FBL04 (his~- ade~-) as start strain, WQM2018, Transketolase-deficient mutant was selected after being UV light and N~+ ions implantation induced mutagenesis in Food Biotechnology Research Laboratory of Tianjin University of Science and Technology. The mutant can accumulate 7.86% D-ribose in fermented broth. Its characteristics were also studied in this paper.