Ex-Vivo Expansion of Both Committed and Pluripotent Hematopoietic Progenitor Cells

摘要

UCB ex-vivo expansion has been proposed as a method to increase the number of cells available for transplantation. We investigated total NCs, CD34+ and CD34+CD38- pluripotent non committed cell fold increase after ex-vivo expansion of CD34+ purified cells in the presence of different human growth factors combinations. Results show that the addition or the withdrawal of IL-3 to the early-acting cytokines flt3-L and TPO, is crucial to influence the total NC fold increase and the ratio of immature versus committed cells. We therefore propose the ezpan-sion of each UCB sample in two aliquots containing different cytokines; this protocol allows high NC proliferation as well as high number of non committed pluripotent cells in the final product.