Barophysiology of Saccharomyces cerevisiae from the aspect of 6,000 gene-expression levels

摘要

Hydrostatic pressure is one of the physical factors that affect cellular physiology. With hydrostatic pressure, a few hundred MPa decreases the viability of yeast cells and a few tens MPa decreases the growth rate. However, there is little information about how hydrostatic pressure affects viability and growth. To understand the effects of hydrostatic pressure, we have used yeast DNA chips and analyzed 6,000 gene-expression levels after four different conditions. A 180MPa treatment, 4 for zero minutes, and 0.1MPa, 25°C for 60min, which was used for recovery, induced the genes of BTN2, HSP26, SSC1, and so oa One of 40MPa, 4°C for 16h, and 0.1MPa, 25°C for 60min, induced the genes of SSA4, HSP12, TIP1, and so oa The many genes induced by these treatments belong to the group of molecular chaperon and protein degradation. The genes of YRO2, PST1, and so on were induced by a 40MPa treatment, 25°C for 2h, but the number of induced genes was not very high. The growing yeast cells under 10MPa, 25°C induced the genes of HFA1, PMD1, and so on. The characteristic genes induced under these conditions were cyclins for the G1/S phase and have a chromosome duplication function. The genes induced by pressure treatment suggest that lethal pressure causes damage to the protein structure, and mild pressure inhibits chromosome duplication.