Osteogenic Potential of Porous #beta#-Tricalcium Phosphate (#beta#-TCP) Combined with Cultured Bone. - Tissue Engineered Bone Using a Biodegradable Material as a Scaffold

摘要

Recently, the tissue engineering approach has widespread attention for regeneration. The present study was undertaken to evaluate whether biodegradable Porous #beta#-Tricalcium Phosphate (#beta# -TCP) can be used as a scaffold for cultured bone marrow cells or not. Marrow cells were obtained from bone shaft of rat femur and cultured in a standard medium for 10 days, then trypsinized to combine cells with ceramics. An additional subculture was done for cells/ceramics composite in a standard medium with the addition of #beta# -glycerophosphate, ascorbic acid and dexamethason. The 20 day subcultured composites were implanted into subcutaneous sites of syngeneic rats. These implants were harvested at 4 and 8 weeks postimplantation, and prepared for the histological analysis. In the histological analysis of composites at 4 weeks postimplantation, active bone formation could be found in the composites. The bone formation was evidenced by active osteoblast lining on the surfaces of bone. At 8 weeks, more extensive bone formation was observed in the composites. These results suggested that beta-TCP could play a role as scaffold of tissueengineered bone derived from marrow cells.