Functional analysis of R2R3-Myb genes In rice

摘要

MYB proteins are transcription factors that contain a domain structurally and functionally related to the DNA binding domain of the oncogene c-myb. MYB proteins in plants have two related helix-turn-helix motifs, the R2 and R3 repeats. To investigatethe roles of R2R3-Myb genes in rice, we have carried out polymerase chain reaction using R2R3 degenerate primers in cDNA libraries made from young flowers, mature flowers, and seed coats. We isolated 35 partial cDNA clones of R2R3-Myl) genes. We chose 12of these clones for further study. Antisense transgenic rice plants were produced to study the function of R2R3-Myb genes.