Improved breeding in camelids through molecular techniques: Development of STR multiplexes for identification and parentage verification

摘要

A size-selected dromedary genomic library was constructed and screened for dinucleotide and trinucleotide STR-loci using polymer (CA)n and (GGT)n probes. Positive clones were sequenced and dromedary specific PCR primers were designed for 16 STR loci.Based on the typing of 110 unrelated animals the number of alíeles, alíele frequencies, heterozygosity, the polymorphism information content and the exclusion probability were calculated for each system. In llamas and alpacas (Lang et al., 1996) equivalent data have been published recently. Taking these and our own results multiplex systems for identification and parentage testing by STR-typing in llamas, alpacas and dromedaries have been established for different detection methods, including silver staining as well as single colour and multicolour fluorescence detection. Allelic ladders characterized by sequence analysis will be prepared for each of the STR loci in these multiplexes. DNA from different sources (blood, saliva, hair) has been compared concerning the quality and quantity of the resulting amplification products. The suitability of these STR loci for identification and parentage verification is discussed.