Solid phase synthesis of seleno-methionine peptides by tBoc/Bzl and Fmoc/tBu protection strategy suitable for the crystallographic phase determination by multi-wavelength anomalous diffraction (MAD)

摘要

The phase determination is one of the central problems for the determination of the electron density map during the X-ray crystallographic analysis of biomolecules.Thus several methods have been developed,which include isomorphous replacement,molecular replacement and multi-wavelength anomalous diffraction (MAD).