Pharmacokinetics of plasmid dna and liposome: plasmid dna complexes after iv bolus administration in the rat.

摘要

The use of viral and non-viral vectors for gene delivery is a rapidly developing area in the pharmaceutical sciences. The rational design of these vectors for gene delivery should be based upon the behavior of naked plasmid DNA (pDNA). Furthermore, the functional form of the plasmid: supercoiled (SC), open circular (OC), or linear (L) is an important parameter to be considered.~1,2 Any differences in transcriptional activity may need to be accounted for in pharmacokinetic/pharmacodynamic models. The purpose of this investigation was to model the pharmacokinetics of naked pDNA and liposome: pDNA complexes in a topoform specific manner after IV bolus administration in the rat.