Direct Raman Measurement of an Elevated Base pK_a in the Active Site of a Small Ribozyme in a Pre-catalytic Conformation

摘要

Raman Spectroscopy of Aqueous AMP Samples We first established the Raman marker bands required to measure the pKa of an N1- imino group of adenine. We subjected a concentrated solution of AMP to a Raman spectroscopic titration analysis. We chose to monitor the 734 cm~(-1)