Development of 1064 nm excited multichannel Raman microspectrometer and its application to in vivo measurements of cyanobacteria

摘要

Near-infrared (NIR) excited Raman microspectroscopy is highly useful for in vivo studies of biological samples, which often show strong fluorescence that interfere the Raman measurements. However, some biological samples emit strong fluorescence even with NIR excitation at —800 nm. For the purpose of further overcoming this difficulty, we have tried to develop a deeper NIR Raman system with the sensitivity high enough for in vivo measurements of single living cells. In this presentation, we report the development of a new 1064 nm deep NIR excited multichannel Raman microspectrometer. Its application to the in vivo Raman measurements of cyanobacteria, which emit strong fluorescence with 800 nm excitation, is also reported.