Cloning of pyrG gene from Aspegillus niger ATCC 12049 and establishment of a heterologous protien expression system

摘要

A. 9.8kb DNA fragment was cloned from genome genome liberary of Aspergillus niger (A.niger) ATCC 12049. Then a 2.3 kb fragment containing pyrG gene was subcloned into a expression plasmid PIGF, getting a recombinant plasmid pYG1.2. The whole pyrG gene was sequenced and analysised, It showed 93.9