Cell wall-affecting antibiotics induce expression of a novel gene drp35 In Staphylococcus aureus

摘要

Staphylococcus aureus has wide range adaptability against stresses and acquires antibiotic resistance rather easily when it is exposed to antibiotics. A certain antibiotics induces a certain resistant factor either form the gene constitutionally encoded on the chromosome or temporally acquired gene from the outside. The methicillin-resistant S. aureus (MRSA), for instance, carries the acquired mecA gene on the chromosome, which encodes a penicillin-binding protein, PBP2'. The expression of the resistance is under the control of the regulators (mecI and mecRI) adjacent to the mecA and panicillinase gene regulators (blaI and blaR1). for the optimal expression methicillin resistance in MRSA, however, it needs PBP2 of which gene located on the other site of the chromosome. The molecular mode of the expression seems to be more complex and sophisticated, another words, it needs coordination of the resistant factor responsible gene and some common house keeping genes. We suspected there should be a common gene that is responsible for antibiotic resistance and have been searching for the candidate gene which was induced with various antibiotics in S. aureus. We report here a novel gene, drp35, which was induced commonly by cell wall-affecting antibiotics but not by the others.