Preparation and epitope mapping of monoclonal antibodies to mex-a protein of mex-a, B/opr-m efflux pump in pseudomonas aeruginosa

摘要

The drug efflux pumps together with outer membrane of lower permeability in Pseudomonas aeruginosa are involved in multi antibiotics resistance of this organism(1). Among several efflux pumps reported in P. aeruginosa, MexA,B-OprM pump has been demonstrated to play an importatn role in the antibiotics resistance(2). The component proteins, MexA, MexB and OprM, have recently been characterized biochemically(3), but their membrane topology and precise localization therein have not been clarified yet. In the present study, we have raised monoclonal antibodies against the MexA protein of the efflux pump and carried out epitope mapping in order to get insight into its membrane topology.