DEVELOPMENT OF CARBON NANOTUBE-BASED ELECTROCHEMICAL BIOCATALYST BY ONE-STEP AS PLATFORM FOR BIOSENSING

摘要

The development of biofunctional electrodes for energy generation under green conditions(neutral pH,room temperature and source of energy)based on Enzymatic biofuel cell(EBFC)systems,and enzyme-based biosensor for detection of human being analytes for medical interest,have been intensively investigated.One important aspect for a proper performance is the effective interaction between the electrode surface and the enzymatic element,providing high sensitivity,high current density and low on-set potential.In this sense,electron-transfer from the reaction between the enzymatic element and its substrate towards the electrode is a triggering factor for the operation of the device.Even though,carbon nanotubes(CNTs)have been considered a promising material for this kind of application,due to the remarkable chemical stability,biocompatibility,and catalytic and electronic properties,functionalization with surface species are required to guarantee a proper immobilization of the element,to reduce the distance or to connect the active-center with the electrode surface.In this work,electrochemical modification of SWCNTs surface with N and P functionalities and encapsulation of a model enzyme(soluble glucose dehydrogenase(GDH)pyrroloquinoline quinone(PQQ)),is carried out in one step to synthesize a bioelectrode with electrocatalytic activity towards glucose oxidation.