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The prevalent strain of maize dwarf mosaic virus in North China is different from SCMV-MDB

机译:华北地区玉米矮状马赛克病毒的普遍存在菌株与SCMV-MDB不同

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Maize dwarf mosaic virus (MDMV) has been reported in many countries. Its epidemics have caused severe quality and yield losses in maize and sorghum since late 1960s in North China. MDMV belongs to Sugarcane mosaic virus (SCMV) subgroup of the genus Potyvirus with a positive-sense ssRNA genome encoding a single polyprotein. A Beijing isolate of MDMV was identified as MDMV-B (now referred as SCMV-MDB) based on its biological properties. Several other isolates from northern China have been identified as the same strain according to their serological and biological properties. The coat protein (CP) gene of the isolate has been cloned and sequenced. Since the deduced protein sequence is different from the conceptual translation, i.e., there are 3 mismatches (Ala at positions 135, 188 and 208 to Phe) and one unexpected amino acid (aa) (Gly instead of Asp at residue in the published CP sequence, further verifying the CP sequence and determining its relationship with SCMV-MDB is needed.
机译:许多国家都报道了玉米矮人马赛克病毒(MDMV)。自20世纪60年代末,其流行病引起了玉米和高粱的严重质量和产量损失。 MDMV属于Potvirus属的甘蔗马赛克病毒(SCMV)亚组,其具有编码单个聚丙烯的正感SSRNA基因组。基于其生物学特性,北京MDMV的分离物被鉴定为MDMV-B(现在称为SCMV-MDB)。根据其血清学和生物学性质,来自中国北部的其他几个其他孤立株已被鉴定为相同的菌株。克隆并测序了分离物的外套蛋白(CP)基因。由于推导的蛋白质序列与概念翻译不同,即,存在3个错配(Ala在位置135,188和208至pHE上)和一个意外的氨基酸(AA)(在公开的CP序列中的残基代替ASP。 ,需要进一步验证CP序列并确定其与SCMV-MDB的关系。

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