A dissection of recombinant protein expression in CHO cells indicating protin-specific, not cell specific, limitation

摘要

The expression of recombinant Component B (rCB) in Chinese hamster ovary (CHO) cells was investigated. No factor (s) limiting expression were identified when the cell-specific parameters of transcription, translation and post-translational modification were investigated. Following this, recombinant growth hormone (uGH) was used as a surrogate marker to demonstrate that the CHO cellular machinery was not limiting the rCB expression. The protein-specific nature of rCB expression was then confirmed when it was found that CHO cells expressing rCB had elevated levels of GRP78 suggesting cellular stress from protein misfolding.