Comparison of immobilised group specific affinity ligands for the bioseparation of antibodies by high performance membrane affinity chromatography

摘要

High Performance Membrane Affinity Chromatography (HPMAC) using Convective Interaction Media (CIM) disks has a high potential as fast multipurpose separation method in downstream processing and Quality Control of biopharmaceuticals [1, 2]. Protein A, protein G and protein L have been immobilised on epoxy groups of poly(glycidyl methacrylate-co-ethylene dimethacrylate) macroporous disks (BIA, Slovenia) and used as affinity chromatographic stationary phases for the separation of human and bovine polyclonal and recombinant monoclonal IgGs. The specificity of the affinity ligands for antibodies (protein A and protein G for Fc fragments [3, 4] and protein L for kappa light chains [5]) allows isolation of IgGs, for example, from cell culture supernatants.