Large scale production of HIV-1 gp120 using recombinant vaccinia virus in packed-bed bioreactor

摘要

The HeLa cell-vaccinia virus system is an attractive method for producing recombinant mammalian proteins with proper post-translation modifications. This system is especially applicable to the production of the HIV-1 envelope glycoprotein, gp 120, because more than half of its total mass is carbohydrates. A recombinant vaccinia virus/T7 RNA polymerase expression system to produce large amounts of gp 120 was developed. The packed-bed bioreactor configuration was examined tbr pilot-scale production and was tbund to be an efficient system tbr both cell growth and virus infection resulting in high levels of gp 120.