DIRECT AND INDIRECT DETECTION OF HAEMOPHILUS PARASUIS AND MYCOPLASMA HYORHINIS AND ASSOCIATION WITH SEROSITIS IN SWINE

摘要

As the indirect detection of Haemophilus parasuis (Hps) infections in pigs does not correlate with clinical and pathologic findings of serositis (1) the impact of a coinfection with Mycoplasma hyorhinis (Mhyorhinis) was analysed. A total of 143 pigs were included in the study. These were turned in for clinical diagnosis. From all pigs blood samples were taken. All animals were examined clinically by auscultation and assigned clinical scores from 1 to 7. Additionally the presence of serositis (peritonitis, pleuritis and pericarditis) was documented pathologically. Collective swabs of the serosal surfaces were sampled and analysed by PCR (2) for HPS and Mhyorhinis. Detection of antibodies were carried out with the Swinechek7 HPS ELISA (Biovet, Quebec, Canada) for the indirect detection of Haemophilus parasuis as described by the manufacturer and with an ELISA performed by the method of Engvall and Perlmann (3) using a whole-cell-preparation of the Mycoplasma hyorhinis type strain as the antigen.