Performance of desulfitobacterium frappieri PCP-1 in a continuous anaerobic reactor treating pentachlorophenol

摘要

In this work, a mixed bacterial community of an anaerobic upflow sludge bed reactor degrading pentachlorophenol (PCP) was augmented in a strain of anaerobic PCP degraders Desulfitobacterium frappieri (strain PCP-1). To estimate the efficiency of augmentation, the population of PCP-1 in the reactor was enumerated using a competitive polymerase chain reaction (cPCR) technique. Stain PCP-1 appeared to compete wellwith other microorganisms of the mixed bacterial community with its population increasing from 10~6 to 10~(10) cell/g volatile suspended soilids over a period of 70 days. Proliferation of the strain PCP-1 allowed for a substantial increase of the volumetric PCP load from 5 to 80 mg/L/day.