Peroxidase Reaction under High Pressure: Influence of Different Hydrogen Donor Molecules

摘要

As a consequence of cell destruction during high pressure treatment, enzymes of fruit are no longer separated from their substrates. Pressure conditions may lead to an acceleration of enzyme reactions or a different affinity to different substrates due to changes in the structure of proteins, so those in the tertiary atructure of enzymes could affect their specifity, which may form or increase concentration of undesirable compounds. Effects of high pressure between 0.1 and 550 MPa on commercial horse-radish peroxidase reaction velocity were measured by spectrophotometric methods. The reactions tested have been peroxidations taking place when hydroquinone or guajacol as hydrogen donors were in the reaction mixture.