Sensitive detection of pesticide using ENFET with enzymes immobilized by cross-linking and entrapment method

摘要

Trichlorfon as a common pesticide is detected using a BuChE-FET sensor which is prepared with cross-linked BASA-glutaraldehyde menmbrane or photocross-linkable PVA/SbQ (poly(vinyl alchol) containing styrylpyidinium) memberane. On the basis of these BuChE-FET sensors, a biochemical test for trichlorfon in liquid was developed by enzyme inihibition. PVA/SbQ memberane shows better characteristics compared to BSA memberane: large linear dynamic range in kinetic mode, better reproducibility, and above all, better stability in storage. Nevertheless, the both membreanes present the same detection limit for trichlorfon (10~(-6) M).