A lactate needle-type biosensor for in vivo detection in muscular tissues

摘要

As an alternative method for whole blood lactate monitoring for clinical and sports medicine,a needle-type lactate biosensor [1] is designed for in vivo detection of lactate in muscular tissues.It is ocnstructed by a stainless steel needle,with the surface modified by a polymer material for the enzyme (lactate oxidase,LOD) immobilization.The influence of pH,temperature,concentration of the enzyme and the immobilization condition are discussed.It cna determine the substrate with the linear range 4.8x10~-6--3.2x10~-3 mol/L,response time <1min.The possible interference of the ascorbic acid,urea,glucose etc.for in vivo detection is studied.The biosensor is used in the detection of the lactate in vivo in muscular tissues of the Wistar rats,and good results are obtained.