The measurement of the phosphorescence and singlet oxygen fluorescence time-resolved waveforms of Photofrin(II) and Talaporfin sodium with pulsed excitation

摘要

In order to investigate the interaction between the triplet state T1 and ground state oxygen 3O2 during pulsed excitation photodynamic therapy (PDT), we measured the phosphorescence and singlet oxygen 1O2 fluorescence time-resolved waveform. The phosphorescence time-resolved waveform from clinical photosensitizers has not been obtained because this signal was buried in the photosensitizer fluorescence. We constructed the experimental setup with a spectral and temporal filter to select the phosphorescence signals from the Photofrin(II)(R) and Talaporfin sodium solution. The lifetimes and spectrums of the measured luminescence coincided with the phosphorescence characteristics, respectively. We obtained the phosphorescence time-resolved waveforms from the clinical photosensitizer solutions successfully. The 1O2 fluorescence time-resolved waveforms from these photosensitizers were measured with an IR-PMT with a photon counter. The fluorescence time-resolved waveforms of each photosensitizer were also obtained by the authors. We could consequently describe sequential generation of three time-resolved waveforms throughout the photosensitive reaction in the clinical photosensitizers. We think we may evaluate the photoseisitizer characteristics by these waveforms.