CHEMILUMINESENCE SINGLE INCUBATION MULTILAYER IMMUNE TECHNIQUE DETECTION OF ROTAVIRUS IN EXCREMENT

摘要

Though application of chemiluminesence detection effectively acheives higher sensitivity, it still restricted by classical ELISA immunoreaction model. Naser successfully acheived 10-20-foid sensitivity by co-incubating the standard bridge antibody and enzyme-linked secondary antibody in ELISA to detect immobile antigen, In our paper, theorical treatment are given. it also proved that sample can also be detected by co-incubation with other standard immunoreagents without washing step. With chemiluminesenee detection system, a new method termed Chemiluminesence Single Incubation Multilayer immune Technique (CLSIMIT) to determinate RV in exerement is proposed.