Investigations concerning the determination of NADH concentrations using optical biopsy

摘要

The intrinsic NADH autofluorescence intensity of biological tissue depends on the local, cellular concentration of this coenzyme. It plays a dominant role in the Krebs-Cycle and therefore serves as indicator for the vitality of the observed cells. Due to individually and locally varying boundary conditions and optical tissue properties, which are scattering coefficients, absorption coefficients and g-factors the fluorescence signal needs to be rescaled. One possible rescaling method is the theoretical derived Photon Migration Theory. Our new rescaling method is partly based on measurements and partly theoretical derived. By using the 4 information channels: LIF time-resolved signal, biochemical concentration measurements, Monte Carlo simulations with optical parameters and microscopic investigations we demonstrate that simultaneous detection of the fluorescence and the backscattering signal can easily and accurately provide rescaled, quantitative values for the NADH concentrations.