Fast Cell Immobilization by Using Non-immunological Method for Cell Based Biosensor

摘要

This paper reports a concept of a micro cell analysis system for fast cell immobilization by using a non-immunological method which is composed of a cell/drug inlet, a sheath flow inlet, a micro chamber and a outlet to biosensor/waste. Micro cell wells were located in the micro chamber. To define optimized parameters of micro cell analysis system, cell adhesion experiment using Hela at 0.5X10~6, 1.0X10~6 or 2.0X10~6 cells/ml in micro wells with lOpm depth and 25μm, 50pm and 100pm size respectively was performed. Different tendencies of cell adhesion between 0.5X10~6 cells/ml and 1.0X10~6 cells/ml concentration were not detected. The highest concentration gave the best performance of cell immobilization, even though cell congregation appeared.