High Throughput Screening System for screening of 3D cell cultures

摘要

Cancer is always an intensively studied field due to an increasing number of incidences. Therapeutic strategies are often associated with negative side effects, which should be minimized. Therefore, natural drugs provide an interesting prospect for cancer therapy by less toxic adverse effects. To test a high number of drugs, automation offers a good approach for the realization of a higher sample throughput. This paper presents a High Throughput Screening System to investigate the effect of different drugs on 3D cell cultures (celisca, Rostock, Germany). We used 3D cell cultures because of their higher similarity to physiological conditions due to the structure. Referring to the High Throughput Screening system, the arrangement is equipped with specific devices to perform a high throughput drug screening. Here, the instrumentation and possibilities of data measurement are described. In conclusion, this screening system could be used in industry for a drug screening in a high throughput manner, which is based on 3D cell cultures.