Auto Nanomanipulation System for Single Cell Stiffness Measurement

摘要

In this paper, an auto nanomanipulation system wasproposed to measure the stiffness of single cell inside an environmentalscanning electron microscopy (ESEM). A nanoneedlewas prepared previously to measure the stiffness of single cell.The needle was fixed to the nanorobotic manipulator, which hasthree degrees of freedom, i.e. X, Y and Z translation. Singleyeast cell was chose as sample and put on an tungsten probesubstrate inside ESEM. Vision feedback method was used toget the position of the nanoneedle and single cell. After that,these information were used to control the movement of thenanorobotic manipulator. Finally, the stiffness of the single cellwas measured using the nanoneedle driven by the automaticnanomanipulation system.