Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues

摘要

The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications suchas retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effectivetechnique in AGEs detection and the establishing the correlation of measured AGE parameters to diabetic pathogenesisare invaluable in the monitoring of disease progression and drug discovery of anti-AGE compounds. Since some AGEare fluorescent, we propose to investigate the degree of tissue glycation in forming fluorescent AGEs (fAGEs). In thispreliminary study, we will investigate the effects of glucose, fructose, and galactose, three of the most abundant dietarysimple sugars, in fAGEs production. Excised tissues will be treated in solutions containing the three sugar types;multiphoton autofluorescence imaging will then be performed on the treated tissues to determine their autofluroescencelevels.