Feature analysis of wrapped phases of nucleated cells in polar coordinates

摘要

Digital phase microscopy has made great progress in the study of morphology and dynamics of biological cells,especially the homogeneous cells. For the heterogeneous nucleated cells with relatively complex internal structures,there are still few methods of phase unwrapping suitable for observation and measurement. In this paper, a method isproposed to identify the substructure of cells only with their wrapped phases. According to the structural features ofreal nucleated cells, their similar models are established firstly, and then their wrapped phases are obtained inCartesian coordinates and polar coordinates. Based on the relationship between the parameters of the two coordinatesystems, the pattern recognition and image processing techniques are used to analyze the parcel phase of thesingle-core and multi-core, regular and irregular kernels, and the central and boundary kernel models. Then, thetexture shape distribution, the texture overlap condition, the opening and closing state of the equal phase line, thepeak number and the peak position are selected as reference features, and these reference features are combined andoptimized as the judgment condition for identifying the nuclear structure model, and the wrapped phase in polarcoordinates is screened and identified, which effectively distinguishes three typical types of nuclear cell models. Itprovides a new, unpacked method for the classification and identification the substructures of the nucleated cells.