Multiphoton imaging of normal breast lobules, pleomorphic invasive lobular carcinoma, and classic invasive lobular carcinoma

摘要

Pleomorphic invasive lobular carcinoma (pILC) is a distinct morphological variant of invasive lobular carcinoma (ILC),which is more aggressive than classic invasive lobular carcinoma (cILC) with worse overall prognosis. It is critical todistinguish between pILC and cILC for management and treatment of patients. Multiphoton microscopy (MPM),basedon two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), produces subcellular resolutionimages to show detailed information on structure and cell morphology of tissue sections without staining. In this work,MPM was introduced for label-free identification of normal breast lobules, pILC and cILC, which is consistent withhaematoxylin and eosin (H&E) staining. Furthermore, analysis of nuclear area is used to quantitatively reflect theircytological characteristic. These results demonstrate that, MPM has the ability to distinguish between normal breastlobules, cILC, and pILC by histopathological features, which can provide additional indicators to determine adequatetreatment strategies of patients with pILC for pathologists and surgeons. In the future, MPM may be used as a diagnostictool of lobular cancer into routine clinical use.