Probing chemosensitivity and energy metabolism in patients-derived colorectal cancer cells

摘要

Colorectal cancer is one of the most commonly diagnosed and poorly responding to chemotherapy types of cancer,which emphasizes the importance of personalized approach to treatment selection. Short-term primary cell culturesestablished from patients’ tumors represent a valuable model for testing drug response. In this study, we developedprotocols for generation of the short-term primary cell cultures from colorectal cancer tissue and assessment theirchemosensitivity using MTT test. Additionally, we showed the possibility of metabolic analysis of patient-derivedcancer cells using fluorescence lifetime imaging (FLIM) of autofluorescent cofactor NAD(P)H. Since FLIM ofNAD(P)H demonstrates the potential to detect early responses to cancer treatment, we assume that this method, alone orin combination with MTT assay, can be used for choosing the optimal chemotherapy for patients.