Design of a real-time laser stimulus system based on MEMS-based confocal laser scanning microscope

摘要

We design a real-time laser stimulus system for laser confocal scanning microscope. By introducing the FPGA and AOMto achieve high speed modulation of a scanning laser, we can adjust the laser lighting area freely. For reducing the size ofthe optical path, we use MEMS-mirror instead of traditional fast and slow axis mirrors. The size of MEMS-mirror is 1.5mm diameter and the scanning frequencies are set 16 kHz and 12 Hz at the fast and slow axis, respectively. Our systemis capable of delivering stabilized large stimulus pattern (up to 500 × 500 pixels) to the biological tissues.