Laser scanning confocal microscope, and the alignment adjusting method of an optical system of a laser scanning confocal microscope

摘要

PROBLEM TO BE SOLVED: To provide a technology enabling confocal scanning means to reciprocally scan a sample without causing any light and dark stripes in an image obtained by a laser scanning type confocal microscope.SOLUTION: A difference between the intensity of light detected by an optical detector during the approaching period of the resonance type scanning means provided for scanning a sample by use of a laser beam through the resonance movement of a mirror and the intensify of light detected by the optical detector during the returning period of the resonance type scanning means (step S3). The relative positional relation between a confocal pinhole and the optical axis of detected light entering the confocal pinhole by rotating a beam shifter 6 is adjusted until the calculated intensity difference becomes equal to or below a predetermined value T (steps S4 and S7).