Water-soluble copolymers have widely used in many applications such as detergents, paints, and chemical intermediates. After used in the industrial process, it is discarded as wastewater. However, most of synthetic water-soluble copolymers are low biodegradable and implies absorption on sewage sludge. In this study, a combined anaerobic-aerobic system consisting of an upflow anaerobic sludge blanket (UASB) reactor and a down-flow hanging sponge (DHS) reactor was applied to the treatment of industrial wastewater containing the low biodegradable water-soluble copolymer. The UASB reactor performed 61 ± 16% of COD removal efficiency with organic loading rate of 2.12 kg-CODm~(-3) day~(-1) . The biogas production and methane recovery ratio of UASB reactor were 19.3 ± 6.7 Lday~(-1) and 61.4 ± 26.9%, respectively. The total system achieved high COD removal of 86 ± 7%. The gel permeation chromatography analysis indicated that the molecular weight of wastewater was mainly reduced in the DHS reactor. Massively 16S rRNA gene sequencing investigated polyethylene glycol degrading bacteria Pelobacter sp. was dominated in the UASB granular sludge. Thus, the proposed UASB-DHS system can be applied for this wastewater.
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