Use of a biphasic perfusion process based on mild hypothermia for recombinant glucocerebrosidase (GBA) production

摘要

O For both cell clones, a subphysiological temperature of 31°C led to an increase in the specific productivity (q_p), but the effect of valeric acid supplementation was clone dependent. 1. The best overall performance regarding both cell growth and productivity was obtained for the CHO-GBA-65P cell clone at 31°C without valeric acid. 2. The maximum product titer achieved in perfusion was 9.5-fold higher compared to batch at 31°C and 22-fold higher than the control batch (at 37°C). 3. Perfusion typically results in higher volumetric productivities but lower titers. However, for this enzyme, our results show that a biphasic perfusion strategy including a temperature downshift can significantly enhance also GBA titer in the harvest, which is an advantage for subsequent downstream processing.