Calcium signaling response of osteoblastic cells received with compressive stimuli

摘要

This paper presents the evaluation of osteoblastic cell calcium signaling responses that received with compressive stimuli by fabricated microdevice. This microdevice was constructed by stacking of two PDMS structural layers. The compressive stimulus to cells can be controlled by regulating the expansion of the diaphragm via a pneumatic pressure to the device. In this experiment, compressive stimulus was gradually applied to the cells from 0-12 kPa. As a result, calcium signaling response of the center of cell and the edge of cell showed different behavior. In addition, when the compressive pressure became the 1.5 kPa, the calcium concentration of edge of cell was drastically increased. These results suggested that there is a threshold for the compressive stimulus received by the cells.