Nanostructured Silicon Coupled with Iron Oxide Magnetic Nanoparticles as Enhanced Desorption/Ionization on Silicon Mass Spectrometry for Phosphopeptide Analysis

摘要

In this paper, we demonstrate using nanostructured silicon (nSi) as desorption/ionization on silicon (DIOS) substrate and coupled with functionalized ioron oxide magnetic nanoparticles (MNPs) for phosphopeptide mass spectrometry (MS) analysis. Phospholyted peptide has been successfully selectively detected by nSi incorporated with TiO_2 and ZnO MNPs. Results show that MNPs coupled nSi MS technique (MNPsSi-MS) is a high performance approach for phosphopeptide analysis where nSi service as DIOS-MS target substrate for high sensitivity MS detection and MNPs can selectively capture the phosphopeptide for enhanced MS detection selectivity.