Effects of UV-B Radiation and NaCl Stress on the Protein Expression in Nostoc flagelliforme

摘要

To establish an efficient SDS-PAGE system to separate proteins of Nostoc flagelliforme cells, the protein extraction, sample volume, and other conditions were modified. The results showed that total proteins were extracted by using acetone as precipitator, the most appropriate sample volume was 30-45 μg, concentration of resolving gel was 12 %, when the protein moved to the resolving gel, the current was changed to 20 mA, With Coomassie brilliant blue staining, 13 clear protein bands appeared in SDS-PAGE gel. UV-B radiation affected the conformation of Nostoc flagelliforme proteins (33, 114, and 130 kDa protein), and salt stress induced 117 and 130 kDa protein, showing these proteins were important factors for the adaption to the corresponding adverse environment.