Experimental systems for optogenetic control of protein activity with photodissociable fluorescent proteins

摘要

Fluorescent Light-inducible Proteins are proteins of interest that have Dronpa domains fused to both termini. In the dark, the Dronpa domains tetramerize and cage the protein, but light induces Dronpa dissociation and activates the protein. Here, we describe four experimental systems for light activation of mammalian cells expressing FLiPs.