DNA Damage Induced in Glioblastoma Cells by 1-131: A Comparison between Experimental Data and Monte Carlo Simulation

摘要

The passage of ionizing radiation in living cells creates clusters of damaged nucleotides in DNA. In this study, DNA strand breaks induced by the beta particle of iodine-131 (1-131), have been determined experimentally and compared to Monte Carlo simulation results as a theoretical method of determining131]! damage. For conducting this experimental study, Glioblastoma (GBM) cells were exposed to 10 mCi 1-131, at a dose of 2 Gy, in order create single strand breaks (SSB) and double strand breaks (DSB) in the DNA of irradiated cells. Cells were evaluated quantitatively by the Fast Micromethod assay. The energy spectrum of electrons released in cells were obtained by the macroscopic Monte Carlo code (MCNP4c) and used as an input of the micro Monte Carlo code (MCDS). The percent of damage induced in cells was calculated by Man vitny test. A significant reduction (p<0.05) in fluorescence intensity in irradiated cells compared to control cells as determined by the Fast Micro method assay represented induced SSB and DSB damages in the DNA of irradiated cells. By comparing the experimental and theoretical results, the difference between the percent of SSB per Gy was about 7.4% and DSB was about 1% per Gy. The differences in experimental and theoretical results may be due to the algorithm of applied codes. Since the Fast Micromethod and other experimental techniques do not provide information about the amount of detailed and complex damages of DNA-like base damages, the applied Monte Carlo codes, due to their capability to predict the amount of detailed damages that occur in the DNA of irradiated cells, can be used in in vitro experiments and radiation protection areas.