Combination of Spectroscopic Methods for Inline Monitoring and Control of Mammalian Cell Cultivations

摘要

The integration of strengths of different spectroscopic methods such as Near Infraredspectroscopy (NIRS), Mid-Infrared spectroscopy (MIRS), 2-Dimensional Fluorescence Spectroscopy(2DFS) and Dielectric Spectroscopy (DS) allowed monitoring and control of different culture attributesto be readily available (inline) instead of being determined in the laboratory (offline). This is anadvantage over analytical laboratory methods, since spectroscopic sensors can enable theintensification of in-process analytics.Moreover, since these spectroscopies are focusing on information of different quality attributes(chemical, physical, physiological) extractable from a biotechnological cultivation process, thecombination of those has the potential to enhance the process understanding significantly. An overviewof our experience in the integration of above techniques - to establish process knowledge in a reducednumber of cultivations for different media, clones and products - will be described.