THE INFLUENCE OF POLYMERIC FIBER STIFFNESS AND ALIGNMENT ON CYTOPLASMIC BLEB DYNAMICS AND MIGRATION OF GLIOBLASTOMA MULTIFORME CELLS

摘要

STEP platform was used to investigate the dynamics of migration and blebbing for highly malignant DBTRG cell line. DBTRG migration was increased with reduced mechanical fiber beam stiffness and the cells were observed to stop blebbing beyond a physical stretch of 1500 μm~2. Our future efforts are devoted towards mapping the fiber stiffness using AFM and calculating the cellular elasticity through the focal adhesion-actin stress fiber network of cells spread on STEP fibers and relate it to the blebbing and migratory behavior to develop a deeper understanding on cancer and cellular dynamics in a tunable mechanistic environment.