A 3D Pt nanoflowers decorated micro-needle tip for in situ detection of cellular ATP release

摘要

Cellular adenosine triphosphate (ATP) release detection is of great importance owing to its signaling function. For increasing the electrode surface area and simultaneously maintaining the enzyme activity, a 3D Pt nanoflowers decorated micro-needle tip electrode has been fabricated through a simple method in this work. A dual enzyme ATP biosensor was developed by the co-immobilization of glucose oxidase and hexokinase on the surface of the 3D Pt nanoflowers decorated micro-needle tip. The simply fabricated ATP biosensor can be used for in situ determination of cellular ATP release with a sensitivity of 0.84 ± 0.10 nA μM~(-1) mm~(-2) to ATP and a low limit of detection (5 μM, S/N = 3). The proposed 3D Pt nanoflowers decorated microelectrodes are promising to be used for the determination of other neurotransmitters and cell secretions.